dc.contributor.author |
SANGEETHA A/P KRISHNANSAMY |
|
dc.date.accessioned |
2017-11-15T05:08:33Z |
|
dc.date.accessioned |
2018-07-10T08:38:52Z |
|
dc.date.available |
2017-11-15T05:08:33Z |
|
dc.date.available |
2018-07-10T08:38:52Z |
|
dc.date.issued |
2017 |
|
dc.identifier.uri |
http://localhost:8080/xmlui/handle/123456789/8929 |
|
dc.description.abstract |
General overview: Neuroinflammation plays an important role in Alzheimer's disease (AD) pathogenesis. Prolonged activation of microglia can contribute to excessive production of pro-inflammatory cytokines. Neuronal stem cells (NSCs), is a potential AD therapy due to its regenerative abilities. Therefore, this study aims to investigate the regulatory effect of NSCs in lipopolysaccharides (LPS)-stimulated BV2 microglia cells.
Methods: The effect of NE4C neuronal stem cell on LPS-stimulated BV2 cells were examined using transwell co-culture system. Real time reverse transcription polymerase chain reaction (qRT-PCR) and western blotting were used to study the regulation of inflammasome receptors expression that regulates the pro-inflammatory protein productions. The tested samples were further investigated for the inhibition of caspase-1 and pro-inflammatory cytokine, interleukin 1β (IL-1β) by testing the supernatants in the co-culture using caspase-1 colorimetric assay and enzyme-linked immunosorbent assay (ELISA) respectively.
Results: Transwell co-culture of LPS-stimulated BV2 cells with NE4C significantly downregulated (p<0.01) mRNA expression of inflammasome receptors. Further studies on inflammasome dependent caspase-1 expression and IL-1β secretion showed NE4C inhibited inflammasome-mediated inflammation by regulating pro-inflammatory cytokines involved in activation of inflammation.
Conclusion: The NE4C inhibits LPS induced neuroinflammation in BV2 cells by suppressing caspase-1 expression and IL-1β secretion through regulation of inflammasome activation. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
International Medical University |
en_US |
dc.subject |
Microglia |
en_US |
dc.subject |
Cell Culture Techniques |
en_US |
dc.subject |
Gene Expression |
en_US |
dc.subject |
Inflammasomes |
en_US |
dc.subject |
DNA |
en_US |
dc.subject |
Alzheimer Disease |
en_US |
dc.title |
THE REGULATION OF INFLAMMASOME ACTIVATION IN BV2 MICROGLIA CELLS BY NEURONAL STEM CELLS (NSCs) |
en_US |
dc.type |
Thesis |
en_US |